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Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.

PUS1 Antibody

CATALOG NUMBER: 28-640

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PUS1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PUS1 antibody can be used for detection of PUS1 by ELISA at 1:312500. PUS1 antibody can be used for detection of PUS1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
SP2/0 Cell Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PUS1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PUS1
Additional Names:
PUS1, MPUS1, mPus1p, A730013B20Rik
Protein Accession Number:
NP_001020733
Protein Gi Number:
70906472
Ncbi Gene Id Number:
56361
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pus1 converts specific uridines to PSI in a number of tRNA substrates. Pus1 acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Pus1 is involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
Background Reference 1:
Zhao, X., et al., (2004) Mol. Cell 15 (4), 549-558.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-640

List Size:
100 ul

List Price:

$519.00

Datasheet
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