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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
PUS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

PUS1 Antibody

CATALOG NUMBER: 56-681

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PUS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human PUS1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PUS1
Additional Names:
tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridine(38-40) synthase, tRNA pseudouridylate synthase I, tRNA-uridine isomerase I, PUS1
Protein Accession Number:
Q9Y606
Protein Gi Number:
114152895
Ncbi Gene Id Number:
80324
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a pseudouridine synthase that convertsuridine to pseudouridine once it has been incorporated into an RNAmolecule. The encoded enzyme may play an essential role in tRNAfunction and in stabilizing the secondary and tertiary structure ofmany RNAs. A mutation in this gene has been linked to mitochondrialmyopathy and sideroblastic anemia. Alternate splicing results inmultiple transcript variants.
Background Reference 1:
Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278(2010)
Background Reference 2:
Sibert, B.S., et al. RNA 14(9):1895-1906(2008)
Background Reference 3:
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Background Reference 4:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-681

List Size:
400 ul

List Price:

$495.00

Datasheet
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