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Western blot analysis of extracts of various cell lines, using PVALB antibody (15-053) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using PVALB antibody (15-053) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PVALB antibody (15-053) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PVALB antibody (15-053) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PVALB antibody (15-053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PVALB Antibody

CATALOG NUMBER: 15-053

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PVALB (NP_002845.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse skeletal muscle
Positive Control 2:
Rat skeletal muscle
Predicted Molecular Weight:
Observed: 13kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PVALB
Additional Names:
PVALB, parvalbumin, HGNC:9704, D22S749, parvalbumin alpha
Ncbi Gene Id Number:
5816
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-053

List Size:
100 uL

List Price:

$436.00

Datasheet
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