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43-303 (0.5ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
43-303 (0.5ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

Pycard Antibody

CATALOG NUMBER: 43-303

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DLTDKLVSYYLES
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 21+24kDa band observed in Mouse Spleen lysates (calculated MW of 21.5kDa according to NP_075747.3). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Predicted Molecular Weight:
Approx 21 + 23kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Pycard
Additional Names:
Pycard, PYD and CARD domain containing, 9130417A21Rik, Asc, CARD5, TMS-1, TNS1, masc, PYD and CARD domain-containing protein, apoptosis-associated speck-like protein containing a CARD
Protein Accession Number:
NP_075747.3
Protein Gi Number:
10801602
Ncbi Gene Id Number:
29108
Background Reference 1:
Compan V, Baroja-Mazo A, López-Castejón G, Gomez AI, Martínez CM, Angosto D, Montero MT, Herranz AS, Bazán E, Reimers D, Mulero V, Pelegrín P.Cell Volume Regulation Modulates NLRP3 Inflammasome Activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-303

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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