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45-129 (0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-129 (4ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-129 (0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PYCARD Antibody

CATALOG NUMBER: 45-129

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RESQSYLVEDLERS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 19kDa band observed in lysates of cell line HeLa (calculated MW of 20.0kDa according to NP_660183.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Positive Control 1:
Cat. No. 1215 - U937 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PYCARD
Additional Names:
PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1
Protein Accession Number:
NP_037390.2,NP_660183.1
Protein Gi Number:
10835256
Ncbi Gene Id Number:
29108
Background Reference 1:
Ohtsuka T, Ryu H, Minamishima YA, Macip S, Sagara J, Nakayama KI, Aaronson SA, Lee SW. ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway. Nat Cell Biol. 2004 Feb;6(2):121-8

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-129

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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