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Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PYGM using rabbit polyclonal PYGM Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

PYGM Antibody

CATALOG NUMBER: 57-778

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PYGM
Additional Names:
Glycogen phosphorylase, muscle form, Myophosphorylase, PYGM
Protein Accession Number:
P11217
Protein Gi Number:
3041717
Ncbi Gene Id Number:
5837
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
Background Reference 1:
Tsoi, S.C., et al., J. Soc. Gynecol. Investig. 10(8):496-502 (2003).
Background Reference 2:
Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002).
Background Reference 3:
Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002).
Background Reference 4:
Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-778

List Size:
400 ul

List Price:

$495.00

Datasheet
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