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46-255 (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-255 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-255 (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Pyruvate carboxylase Antibody

CATALOG NUMBER: 46-255

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-KFKEVKKAYVEANQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1 and NP_071504.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Specificity:
All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PC
Additional Names:
PC, pyruvate carboxylase, PCB
Protein Accession Number:
NP_000911.2,NP_001035806.1,NP_071504.2
Protein Gi Number:
106049295
Ncbi Gene Id Number:
5091
Background Reference 1:
St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I. Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme. Science. 2007 Aug 24;317(5841):1076-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-255

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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