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45-107 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-107 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Pyruvate Dehydrogenase Antibody

CATALOG NUMBER: 45-107

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KSFKANLENPIRLA
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDHX
Additional Names:
PDHX, pyruvate dehydrogenase complex, component X, E3BP, OPDX, PDX1, proX, DLDBP, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X, dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, pyruvate dehydrogenase complex, E3-binding protein subunit, pyruvate dehydrogenase protein X component, mitochondrial
Protein Accession Number:
NP_003468.2,NP_001128496.1,NP_001159630.1
Protein Gi Number:
4505699
Ncbi Gene Id Number:
8050
Background Reference 1:
Li Y, Cao X, Li LX, Brubaker PL, Edlund H, Drucker DJ. {beta}-Cell Pdx1 Expression Is Essential for the Glucoregulatory, Proliferative, and Cytoprotective Actions of Glucagon-Like Peptide-1. Diabetes. 2005 Feb;54(2):482-91.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-107

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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