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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).

QTRT1 Antibody

CATALOG NUMBER: 59-309

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This QTRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-371 amino acids from the C-terminal region of human QTRT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
QTRT1
Additional Names:
Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT
Protein Accession Number:
Q9BXR0
Protein Gi Number:
239938842
Ncbi Gene Id Number:
81890
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29)synthesizes queuosine (Q), which is found in tRNAs that recognizeNAU and NAC codons, encoding tyr, asn, asp, and his. ProkaryoticTGT is a single protein of 43 kD. In contrast, mammalian TGTappears to be a heterodimer consisting of a 60-kD subunit (USP14;MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande andKatze, 2001 [PubMed 11255023]).
Background Reference 1:
Chen, Y.C., et al. RNA 16(5):958-968(2010)
Background Reference 2:
Deshpande, K.L., et al. Gene 265 (1-2), 205-212 (2001) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-309

List Size:
400 ul

List Price:

$495.00

Datasheet
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