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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
QTRTD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of QTRTD1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

QTRTD1 Antibody

CATALOG NUMBER: 55-993

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This QTRTD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-350 amino acids from the C-terminal region of human QTRTD1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
QTRTD1
Additional Names:
Queuine tRNA-ribosyltransferase subunit QTRTD1 {ECO:0000255, HAMAP-Rule:MF_03043}, 24229 {ECO:0000255, HAMAP-Rule:MF_03043}, Queuine tRNA-ribosyltransferase domain-containing protein 1 {ECO:0000255, HAMAP-Rule:MF_03043}, QTRTD1 {ECO:0000255, HAMAP-Rule:MF_03043}
Protein Accession Number:
Q9H974
Protein Gi Number:
74752736
Ncbi Gene Id Number:
79691
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).
Background Reference 1:
Chen, Y.C., et al. RNA 16(5):958-968(2010) Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-993

List Size:
400 ul

List Price:

$495.00

Datasheet
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