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46-256 (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-256 (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quaking Antibody

CATALOG NUMBER: 46-256

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: ETKEKPKPTPDY-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in mouse brain lysates (calculated MW of 35.8kDa according to NP_068681.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
QKI
Additional Names:
QKI, quaking homolog, KH domain RNA binding, DKFZp586I0923, Hqk, QK, QK3 , RNA binding protein HQK, homolog of mouse quaking QKI (KH domain RNA binding protein), quaking homolog, KH domain RNA binding
Protein Accession Number:
NP_006766.1,NP_996735.1,NP_996736.1,NP_996737.1
Protein Gi Number:
45827706
Ncbi Gene Id Number:
9444
Background Reference 1:
Wu JI, Reed RB, Grabowski PJ, Artzt K. Function of quaking in myelination: regulation of alternative splicing. Proc Natl Acad Sci USA. 2002 Apr 2;99(7):4233-8. Epub 2002 Mar 26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-256

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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