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Western blot analysis of MKP3 using rabbit polyclonal MKP3-His6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MKP3 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MKP3 using rabbit polyclonal MKP3-His6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MKP3 gene.

R MKP3 Antibody

CATALOG NUMBER: 63-473

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
This Rat MKP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~381 of rat MKP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by dialysis against PBS
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Dusp6
Additional Names:
Dual specificity protein phosphatase 6, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3, Dusp6, Mkp3
Protein Accession Number:
Q64346
Ncbi Gene Id Number:
116663
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis
Background Reference 1:
Muda,M., et al. J. Biol. Chem. 271 (8), 4319-4326 (1996)
Background Reference 2:
Mourey,R.J., et al. J. Biol. Chem. 271 (7), 3795-3802 (1996)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-473

List Size:
400 ul

List Price:

$495.00

Datasheet
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