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45-130 staining (2ug/ml) of mouse heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing RAB11A and probed with 45-130 (mock transfection in first lane).
45-130 staining (2ug/ml) of mouse heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

RAB11A Antibody

CATALOG NUMBER: 45-130

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: TENKPKVQCCQNI
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in Mouse Heart lysates (calculated MW of 24.4kDa according to NP_004654.1). In transfected HEK293 transiently expressing RAB11A a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3ug/ml.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RAB11A
Additional Names:
RAB11A, YL8, RAB11A, member RAS oncogene family, RAB 11A, member oncogene family, MGC1490, Ras-related protein Rab-11A
Protein Accession Number:
NP_004654.1
Protein Gi Number:
4758984
Ncbi Gene Id Number:
8766
Background Reference 1:
Gromov PS, Celis JE, Hansen C, Tommerup N, Gromova I, Madsen P. Human rab11a: transcription, chromosome mapping and effect on the expression levels of host GTP-binding proteins. FEBS Lett 1998 Jun 16;429(3):359-64

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-130

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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