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Western blot analysis of extracts of various cell lines, using RAB11FIP1 antibody (23-813) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using RAB11FIP1 antibody (23-813) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

RAB11FIP1 Antibody

CATALOG NUMBER: 23-813

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 330-540 of human RAB11FIP1 (NP_079427.4).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
HeLa
Positive Control 2:
HepG2
Positive Control 3:
COS-7
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RAB11FIP1
Additional Names:
RAB11FIP1, RAB11 family interacting protein 1 (class I), HGNC:30265, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1, Rab coupling protein, Rab effector protein, Rab-interacting recycling protein, Rab11-family interacting protein 1D, no Rab11-binding protein 1A
Ncbi Gene Id Number:
80223
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-813

List Size:
100 uL

List Price:

$436.00

Datasheet
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