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Western blot analysis of extracts of various cell lines, using RAB24 antibody (23-551) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver damage using RAB24 antibody (23-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using RAB24 antibody (23-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using RAB24 antibody (23-551) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug RAB24 antibody (23-551). Western blot was performed from the immunoprecipitate using RAB24 antibody (23-551) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using RAB24 antibody (23-551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.

RAB24 Antibody

CATALOG NUMBER: 23-551

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB24 (NP_001026847.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
HT-1080
Positive Control 2:
MCF7
Positive Control 3:
SH-SY5Y
Positive Control 4:
293T
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 23kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RAB24
Additional Names:
ras-related protein Rab-24
Ncbi Gene Id Number:
53917
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-551

List Size:
100 uL

List Price:

$436.00

Datasheet
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