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Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.
Immunohistochemical analysis of paraffin - embedded human bladder carcinoma (top), human breast carcinoma (middle), and human skin carcinoma (bottom) showing cytoplasmic localization using Rab25 antibody with DAB staining.
Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.

Rab25 Antibody [3F12F3]

CATALOG NUMBER: 32-208

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Rab25 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAB25
Additional Names:
CATX8, CATX-8CATX-8, RAB11C
Protein Accession Number:
P57735
Protein Gi Number:
46577696
Ncbi Gene Id Number:
57111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Background Reference 1:
JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422
Background Reference 2:
Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-208

List Size:
0.1 mg

List Price:

$515.00

Datasheet
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