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Western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).
RAB3D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).

RAB3D Antibody

CATALOG NUMBER: 56-924

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RAB3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the C-terminal region of human RAB3D.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAB3D
Additional Names:
Ras-related protein Rab-3D, RAB3D, GOV, RAB16
Protein Accession Number:
O95716
Ncbi Gene Id Number:
9545
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RAB3D is a protein transport. Probably involved in regulated exocytosis (By similarity).
Background Reference 1:
Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010)
Background Reference 2:
Knop, M., et al. EMBO J. 23(15):2982-2992(2004)
Background Reference 3:
Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004)
Background Reference 4:
Nguyen, D., et al. J. Cell. Physiol. 197(3):400-408(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-924

List Size:
400 ul

List Price:

$495.00

Datasheet
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