Rabbit Anti-Chicken IgY (H&L) Antibody (Alkaline Phosphatase)

CATALOG NUMBER: XR-9180

Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Chicken
Conjugate:
Alkaline Phosphatase
Specifications
Host Species:
Rabbit
Species Reactivity:
Chicken
Immunogen:
Chicken IgY - whole molecule
Conjugate:
Alkaline Phosphatase
Tested Applications:
ELISA
Application Note:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).
Purification:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.
Isotype:
IgY
physical-state:
Liquid
Buffer:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)
Storage Conditions:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 °C before opening. DO NOT FREEZE. This product is stable at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XR-9180

List Size:
1 mg

List Price:

$556.00

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