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Western blot analysis of lysates from wild type (WT)293F cells, 293F cells transfected with Snail-His-tag and 293F cells transfected with CYP1A2-His-tag using Rabbit anti His-tag mAb at 1:11000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 10 s.
Immunofluorescence analysis of 293T-SOST His(C) and 293T cells using Rabbit anti His-tag mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg His-tag antibody. Western blot was performed from the immunoprecipitate using His-tag antibody at a dilution of 1:30000.
Western blot analysis of lysates from wild type (WT)293F cells, 293F cells transfected with Snail-His-tag and 293F cells transfected with CYP1A2-His-tag using Rabbit anti His-tag mAb at 1:11000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 10 s.

Rabbit anti His-tag Recombinant Antibody (84-521)

CATALOG NUMBER: 84-521

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Species independent
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Species independent
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IP, WB
Application Note:
WB,1:10000 - 1:60000, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
His
Additional Names:
His;His tag;His-tag
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-521

List Size:
100 uL

List Price:

$326.00

Datasheet
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