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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with mCherry using Rabbit anti mCherry-Tag mAb at 1:20000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 45s.
Immunoprecipitation of CT55-mCherry from 300 µg extracts of 293F cells transfected with a CT55 expression vector containing a single C-terminal mCherry-Tag was performed using 3 µg of Rabbit anti mCherry-Tag mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti mCherry-Tag mAb at a dilution of 1:6000.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with mCherry using Rabbit anti mCherry-Tag mAb at 1:20000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 45s.

Rabbit anti mCherry-Tag Recombinant Antibody (84-537)

CATALOG NUMBER: 84-537

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Species independent
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Species independent
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:10000 - 1:40000, IP,0.5μg-4μg antibody for 200μg-600μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
mCherry
Additional Names:
mCherry; mCherry tag; mCherry-tag
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-537

List Size:
100 uL

List Price:

$326.00

Datasheet
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