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Western blot analysis of lysates from Purified Mouse IgG3κ Isotype Ctrl Antibody using Rabbit anti-Mouse IgG3 (Fc) mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10 ng per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90 s.
Flow cytometry: 1X10^6 THP-1 cells (negative control,left) and BALB/c mouse splenocytes (right) were surface-stained with Purified anti-mouse I-Ad Antibody (2 μg/mL,orange line) or Mouse IgG isotype control (2 μg/mL,blue line), followed by Rabbit anti-Mouse IgG3 (Fc) mAb and Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 BALB/c mouse splenocytes were surface-stained with Mouse IgG isotype control (2 μg/mL,left) or Purified anti-mouse I-Ad Antibody (2 μg/mL,right). Rabbit anti-Mouse IgG3 (Fc) mAb and Alexa Fluor® 647 conjugated goat anti-rabbit pAb was used as a secondary antibody.
Western blot analysis of lysates from Purified Mouse IgG3κ Isotype Ctrl Antibody using Rabbit anti-Mouse IgG3 (Fc) mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10 ng per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90 s.

Rabbit anti-Mouse IgG3 (Fc) Recombinant Antibody (84-799)

CATALOG NUMBER: 84-799

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:1000 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
36kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IgG3
Protein Accession Number:
P03987-2
Ncbi Gene Id Number:
/
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-799

List Size:
100 uL

List Price:

$87.00

Datasheet
Shipping Info

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