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Western blot analysis of lysed extracts from 293 and HeLa cells using Rac1 (Ab-71).
Immunofluorescence staining of methanol-fixed HeLa cells using Rac1 (Ab-71).
Western blot analysis of lysed extracts from 293 and HeLa cells using Rac1 (Ab-71).

Rac1 (Ab 71) Antibody

CATALOG NUMBER: 79-580

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rac1 (Ab-71) antibody was raised against a peptide sequence around aa.69~73 (P-L-S-Y-P) derived from Human Rac1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Rac1 protein.
Predicted Molecular Weight:
21 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RAC1
Additional Names:
MIG5, Rac-1, TC-25, p21-Rac1, Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1
Protein Accession Number:
NP_008839.2
Protein Gi Number:
9845511
Ncbi Gene Id Number:
5879
Background:
Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Aoki K, et al. (2005) Mol Biol Cell; 16 (5): 2207–2217. Kwon T, et al. (2000) J Biol Chem; 275 (1): 423-428

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-580

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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