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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

RACGAP1 Antibody

CATALOG NUMBER: 27-437

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RACGAP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RACGAP1 antibody can be used for detection of RACGAP1 by ELISA at 1:1562500. RACGAP1 antibody can be used for detection of RACGAP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
71 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RACGAP1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RACGAP1
Additional Names:
RACGAP1, CYK4, ID-GAP, HsCYK-4, MgcRacGAP
Protein Accession Number:
NP_037409
Protein Gi Number:
21361397
Ncbi Gene Id Number:
29127
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
Background Reference 1:
Niiya, F., et al., (2005) J. Biol. Chem. 280 (43), 36502-36509.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-437

List Size:
100 ul

List Price:

$473.00

Datasheet
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