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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
RACGAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of RACGAP1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

RACGAP1 Antibody

CATALOG NUMBER: 55-862

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RACGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human RACGAP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RACGAP1
Additional Names:
Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolog, CYK4, HsCYK-4, RACGAP1 (HGNC:9804)
Protein Accession Number:
Q9H0H5
Protein Gi Number:
74762727
Ncbi Gene Id Number:
29127
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rho GTPases control a variety of cellular processes. Thereare 3 subtypes of Rho GTPases in the Ras superfamily of small Gproteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), andCDC42 (MIM 116952). GTPase-activating proteins (GAPs) bindactivated forms of Rho GTPases and stimulate GTP hydrolysis.Through this catalytic function, Rho GAPs negatively regulateRho-mediated signals. GAPs may also serve as effector molecules andplay a role in signaling downstream of Rho and other Ras-likeGTPases.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 3:
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009)
Background Reference 4:
Simon, G.C., et al. EMBO J. 27(13):1791-1803(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-862

List Size:
400 ul

List Price:

$495.00

Datasheet
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