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Western blot analysis of RAD23A Antibody in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of RAD23A Antibody in HL60 cell line lysates (35ug/lane)

RAD23A Antibody

CATALOG NUMBER: 61-127

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This RAD23A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the C-terminal region of human RAD23A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAD23A
Additional Names:
UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A
Protein Accession Number:
P54725
Protein Gi Number:
1709983
Ncbi Gene Id Number:
5886
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RAD223A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
Background Reference 1:
Mueller, T.D., et al., EMBO J. 22(18):4634-4645 (2003).
Background Reference 2:
Mueller, T.D., et al., J. Mol. Biol. 319(5):1243-1255 (2002).
Background Reference 3:
Elder, R.T., et al., Nucleic Acids Res. 30(2):581-591 (2002).
Background Reference 4:
Chen, L., et al., EMBO Rep. 2(10):933-938 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-127

List Size:
400 ul

List Price:

$495.00

Datasheet
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