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Western blot analysis of extracts of various cell lines, using RAD50 antibody (13-069) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human breast cancer using RAD50 Antibody (13-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RAD50 Antibody (13-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody (13-069) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RAD50 antibody (13-069) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

RAD50 Antibody

CATALOG NUMBER: 13-069

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human RAD50
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
NIH3T3
Positive Control 2:
MCF-7
Positive Control 3:
A-549
Positive Control 4:
HT-29
Positive Control 5:
Mouse lung
Predicted Molecular Weight:
Observed: 180kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RAD50
Additional Names:
DNA repair protein RAD50, hRAD50, NBSLD, RAD502, hRad50
Ncbi Gene Id Number:
10111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-069

List Size:
100 uL

List Price:

$436.00

Datasheet
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