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Western blot analysis of extracts of various cell lines, using RAD51C antibody (22-588) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using RAD51C antibody (22-588) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RAD51C antibody (22-588) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using RAD51C antibody (22-588) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RAD51C antibody (22-588) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

RAD51C Antibody

CATALOG NUMBER: 22-588

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RAD51C (NP_002867.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
SW480
Positive Control 3:
BT-474
Predicted Molecular Weight:
Observed: 42kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RAD51C
Additional Names:
RAD51C, RAD51 homolog C (S. cerevisiae), RAD51L2, yeast RAD51 homolog 3, DNA repair protein RAD51 homolog 3
Ncbi Gene Id Number:
5889
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-588

List Size:
100 uL

List Price:

$436.00

Datasheet
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