RAD51D Antibody

CATALOG NUMBER: 46-261

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: GVLRVGLCPGLTEE
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65kDa band in Human Brain and A431 lysates at 1ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of between 38kDa and 6kDda according to NP_002869; NP_598330; NP_598331; NP_598332; NP_598333. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise all three human isoforms of this protein.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RAD51L3
Additional Names:
RAD51L3, RAD51-like 3 (S. cerevisiae), TRAD, R51H3, HsTRAD, RAD51D, recombination repair protein, DNA repair protein RAD51 homolog 4, Trad, RAD51-like 3
Protein Accession Number:
NP_002869.3,NP_598332.1,NP_001136043.1
Protein Gi Number:
217416415
Ncbi Gene Id Number:
5892
Background Reference 1:
Pittman DL, Weinberg LR, Schimenti JC. Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene. Genomics. 1998 Apr 1;49(1):103-11.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-261

List Size:
0.1 mg

List Price:

$369.00

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