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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

RAD54 Antibody

CATALOG NUMBER: 56-150

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This RAD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 634-663 amino acids from the C-terminal region of human RAD54.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAD54L
Additional Names:
DNA repair and recombination protein RAD54-like, 364-, RAD54 homolog, hHR54, hRAD54, RAD54L, RAD54A
Protein Accession Number:
Q92698
Protein Gi Number:
51316508
Ncbi Gene Id Number:
8438
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the DEAD-likehelicase superfamily, and shares similarity with Saccharomycescerevisiae Rad54, a protein known to be involved in the homologousrecombination and repair of DNA. This protein has been shown toplay a role in homologous recombination related repair of DNAdouble-strand breaks. The binding of this protein to double-strandDNA induces a DNA topological change, which is thought tofacilitate homologous DNA paring, and stimulate DNA recombination.Alternative splicing results in multiple transcript variantsencoding the same protein.
Background Reference 1:
Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Background Reference 2:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Background Reference 3:
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 4:
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-150

List Size:
400 ul

List Price:

$495.00

Datasheet
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