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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in IHC on Human Adult Liver at 1:100.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

RAD54B Antibody

CATALOG NUMBER: 25-356

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD54B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RAD54B antibody can be used for detection of RAD54B by ELISA at 1:1562500. RAD54B antibody can be used for detection of RAD54B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
103 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RAD54B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RAD54B
Additional Names:
RAD54B, FSBP, RDH54
Protein Accession Number:
NP_036547
Protein Gi Number:
6912622
Ncbi Gene Id Number:
25788
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
Background Reference 1:
Brys, M., (2007) Pol J Pathol 58 (1), 3-6.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-356

List Size:
100 ul

List Price:

$519.00

Datasheet
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