Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis in 293 cell line lysates (35ug/lane).
Rad9 Antibody (S387) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry.
Confocal immunofluorescent analysis of Rad9 Antibody (S387) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in 293 cell line lysates (35ug/lane).

Rad9 Antibody

CATALOG NUMBER: 55-891

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This Rad9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-391 amino acids from human Rad9.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAD9A
Additional Names:
Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A
Protein Accession Number:
Q99638
Protein Gi Number:
74717382
Ncbi Gene Id Number:
5883
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product is highly similar to Schizosaccharomycespombe rad9, a cell cycle checkpoint protein required for cell cyclearrest and DNA damage repair in response to DNA damage. Thisprotein is found to possess 3' to 5' exonuclease activity, whichmay contribute to its role in sensing and repairing DNA damage. Itforms a checkpoint protein complex with RAD1 and HUS1. This complexis recruited by checkpoint protein RAD17 to the sites of DNAdamage, which is thought to be important for triggering thecheckpoint-signaling cascade. Use of alternative polyA sites hasbeen noted for this gene.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)
Background Reference 3:
Greer Card, D.A., et al. J. Biol. Chem. 285(20):15653-15661(2010)
Background Reference 4:
Bai, H., et al. DNA Repair (Amst.) 9(5):478-487(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-891

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369