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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti in Hela cell line lysates (35ug/lane)
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.

RAD9 Antibody

CATALOG NUMBER: 62-262

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This RAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human RAD9.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAD9A
Additional Names:
Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A
Protein Accession Number:
Q99638
Protein Gi Number:
74717382
Ncbi Gene Id Number:
5883
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.
Background Reference 1:
Maniwa, Y., et al., Cancer 103(1):126-132 (2005).
Background Reference 2:
Wang, W., et al., Proc. Natl. Acad. Sci. U.S.A. 101(48):16762-16767 (2004).
Background Reference 3:
Lindsey-Boltz, L.A., et al., (er) Nucleic Acids Res. 32(15):4524-4530 (2004).
Background Reference 4:
Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-262

List Size:
400 ul

List Price:

$495.00

Datasheet
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