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46-263 (0.3ug/ml) staining of A431cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-263 (5ug/ml) as the reporter antibody.
46-263 (0.3ug/ml) staining of A431cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RAD9A Antibody

CATALOG NUMBER: 46-263

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-QGPSPVLAEDSEGE
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8;274(2):567-70.; PMID: 9872989). Recommended concentration: 0.3-1ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant RAD9A captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RAD9A
Additional Names:
RAD9A, RAD9 homolog A (S. pombe) , RAD9 , RAD9 (S. pombe) homolog, RAD9 homolog, RAD9 homolog (S. pombe), cell cycle checkpoint control protein
Protein Accession Number:
NP_004575.1
Protein Gi Number:
4759022
Ncbi Gene Id Number:
5883
Background Reference 1:
Brandt PD, Helt CE, Keng PC, Bambara RA. The Rad9 protein enhances survival and promotes DNA repair following exposure to ionizing radiation. Biochem Biophys Res Commun. 2006 Aug 18;347(1):232-7. Epub 2006 Jun 21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-263

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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