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46-264 (0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-264 (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-264 (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-264 (0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RAE1 Antibody

CATALOG NUMBER: 46-264

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NAAEELKPRNKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
Specificity:
NP_003601and NP_001015885 represent identical proteins.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RAE1
Additional Names:
RAE1, RAE1 RNA export 1 homolog (S. pombe) , HGNC:9828, FLJ30608, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1, RAE1 (RNA export 1, S.pombe) homolog, homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer), mRNA export protein, mRNA-associated protein MRNP 41, mRNA-binding protein, 41-kD, migration-inducing gene 14, RP4-800J21.2, MGC117333, MGC126076, MGC126077, OTTHUMP00000166110
Protein Accession Number:
NP_003601.1,NP_001015885.1
Protein Gi Number:
4506399
Ncbi Gene Id Number:
8480
Background Reference 1:
Blower MD, Nachury M, Heald R, Weis K. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell. 2005 Apr 22;121(2):223-34.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-264

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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