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Western blot analysis of lysed extracts from HeLa, K562 and HT29 cells using Raf1 (Ab-259).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Ab-259).
Western blot analysis of lysed extracts from HeLa, K562 and HT29 cells using Raf1 (Ab-259).

Raf1 (Ab 259) Antibody

CATALOG NUMBER: 79-257

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Raf1 (Ab-259) antibody was raised against a peptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total Raf1 protein.
Predicted Molecular Weight:
73 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RAF1
Additional Names:
NS5, CRAF, Raf-1, c-Raf, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, C-RAF, C-Raf
Protein Accession Number:
NP_002871.1
Protein Gi Number:
4506401
Ncbi Gene Id Number:
5894
Background:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Background Reference 1:
Dougherty M K, et al. (2005) Mol Cell. 17 (2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2): 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183. Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-257

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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