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Western blot analysis of lysed extracts from MDA cells untreated or treated with PMA for the indicated times using Raf1 (Phospho-Ser338).
Western blot analysis of lysed extracts from MDA cells untreated or treated with PMA for the indicated times using Raf1 (Phospho-Ser338).

Raf1 (phospho Ser338) Antibody

CATALOG NUMBER: 79-472

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Raf1 (Phospho-Ser338) antibody was raised against a peptide sequence around phosphorylation site of serine 338 (R-D-S (p) -S-Y) derived from Human RAF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Raf1 only when phosphorylated at serine 338.
Predicted Molecular Weight:
73 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RAF1
Additional Names:
NS5, CRAF, Raf-1, c-Raf, JAK1A, JAK1B, C-RAF, C-Raf
Protein Accession Number:
NP_002871.1
Protein Gi Number:
4506401
Ncbi Gene Id Number:
5894
Background:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Background Reference 1:
Adams DG, et al. (2005) J Biol Chem. 280 (52): 42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306 (4): 924-9. Noser JA, et al. (2007) Mol Ther. 15 (8): 1531-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-472

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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