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<b> Flow-cytometry using the Anti-RAGE antibody 2A11 ( </b> A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- shaded line) or the r version of 2A11 (24-660- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of A549 cells using anti-RAGE antibody (2A11. </b> Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric r version of 2A11 (24-660) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-660- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-RAGE antibody 2A11 ( </b> Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 2A11 (24-660) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow-cytometry using the Anti-RAGE antibody 2A11 ( A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- shaded line) or the r version of 2A11 (24-660- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

RAGE Antibody [2A11], Rabbit IgG

CATALOG NUMBER: 24-660

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC, Surface Plasma Resonance, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
Human RAGE protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, Surface Plasma Resonance, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
AGER
Additional Names:
AGER, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end products
Ncbi Gene Id Number:
177
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-660

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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