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45-133 (0.5ug/ml) staining of A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-133 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained using a previous batch.
45-133 (0.5ug/ml) staining of A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RAN Antibody

CATALOG NUMBER: 45-133

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: AAQGEPQVQFKLV-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in lysates of cell lines A431, HeLa, HepG2 and NIH3T3 (calculated MW of 24.4kDa according to NP_006316.1). Recommended for use at 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RAN
Additional Names:
RAN, TC4, ARA24, RAN, member RAS oncogene family, Gsp1, OK/SW-cl.81, RanGTPase, guanosine triphosphatase Ran, member RAS oncogene family, ras-related nuclear protein
Protein Accession Number:
NP_006316.1
Protein Gi Number:
5453555
Ncbi Gene Id Number:
5901
Background Reference 1:
Ren M, Drivas G, D'Eustachio P, Rush MG. Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis. J Cell Biol. 1993 Jan;120(2):313-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-133

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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