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45-134 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-134 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-134 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

RANBP1 Antibody

CATALOG NUMBER: 45-134

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-LSVKEETKEDAEEKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in lysate of cell line HeLa (calculated MW of 23.3kDa according to NP_002873.1). Recommended concentration: 0.05-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration: 3-5ug/ml.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RANBP1
Additional Names:
RANBP1, RAN binding protein 1, HTF9A, MGC88701
Protein Accession Number:
NP_002873.1
Protein Gi Number:
4506407
Ncbi Gene Id Number:
5902
Background Reference 1:
Bischoff FR, Krebber H, Smirnova E, Dong W, Ponstingl H. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J. 1995 Feb 15;14(4):705-15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-134

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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