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Western blot analysis of RANBP10 in human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of RANBP10 in human skeletal muscle tissue with RANBP10 antibody at 5 μg/mL.
Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 μg/mL.
Western blot analysis of RANBP10 in human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RANBP10 Antibody

CATALOG NUMBER: 5665

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Mouse: (87%)
Immunogen:
RANBP10 antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10.
The immunogen is located within amino acids 380 - 430 of RANBP10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
RANBP10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RANBP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RANBP10
Additional Names:
RANBP10 Antibody: KIAA1464, Ran-binding protein 10, RanBP10
Protein Accession Number:
EAW83167
Protein Gi Number:
119603573
Ncbi Gene Id Number:
57610
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RANBP10 Antibody: The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.
Background Reference 1:
Wang D, Zaibo Li, Schoen SR, et al. A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/ERK signaling. Biochem. Biophys. Res. Comm.2004; 313:320-6.
Background Reference 2:
Schulze H, Dose M, Korpal M, et al. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J. Biol. Chem.2008; 283:14109-19.
Background Reference 3:
Dasso M. The Ran GTPase: theme and variations. Curr. Biol.2002; R502-8.
Background Reference 4:
Kunert S, Meyer I, Fleischhauer S, et al. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood2009; 114:5532-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5665

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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