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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of RANGAP1 using rabbit polyclonal RANGAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.
RANGAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela cell line lysates (35ug/lane).

RANGAP1 Antibody

CATALOG NUMBER: 57-264

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human RANGAP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RANGAP1
Additional Names:
Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD
Protein Accession Number:
P46060
Protein Gi Number:
1172922
Ncbi Gene Id Number:
5905
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RanGAP1, is a homodimeric 65-kD polypeptide thatspecifically induces the GTPase activity of RAN, but not of RAS byover 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, aregulator molecule that keeps RAN in the active, GTP-bound state.The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequenceis unrelated to that of GTPase activators for other RAS-relatedproteins, but is 88% identical to Fug1, the murine homolog of yeastRna1p. RanGAP1 and RCC1 control RAN-dependent transport between thenucleus and cytoplasm. RanGAP1 is a key regulator of the RANGTP/GDP cycle.
Background Reference 1:
Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)
Background Reference 2:
Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-264

List Size:
400 ul

List Price:

$495.00

Datasheet
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