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Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Western blot analysis in mouse spleen tissue lysates (35ug/lane).

RAP1A Antibody

CATALOG NUMBER: 56-753

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat, Chicken, Zebrafish, Human, Monkey, Xenopus
Immunogen:
This RAP1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-170 amino acids from the C-terminal region of human RAP1A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAP1A
Additional Names:
Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1
Protein Accession Number:
P62834
Protein Gi Number:
51338607
Ncbi Gene Id Number:
5906
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the family ofRAS-related proteins. These proteins share approximately 50% aminoacid identity with the classical RAS proteins and have numerousstructural features in common. The most striking difference betweenRAP proteins and RAS proteins resides in their 61st amino acid:glutamine in RAS is replaced by threonine in RAP proteins. Theproduct of this gene counteracts the mitogenic function of RASbecause it can interact with RAS GAPs and RAF in a competitivemanner. Two transcript variants encoding the same protein have beenidentified for this gene.
Background Reference 1:
Liu, C., et al. Mol. Cell. Biol. 30(16):3956-3969(2010)
Background Reference 2:
Kelly, P., et al. J. Biol. Chem. 285(21):15777-15785(2010)
Background Reference 3:
Ahmed, S.M., et al. J. Biol. Chem. 285(9):6538-6551(2010)
Background Reference 4:
Hsu, Y.H., et al. PLoS Genet.(6), E1000977 (2010) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-753

List Size:
400 ul

List Price:

$495.00

Datasheet
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