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46-267 (1ug/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-267 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-267 (1ug/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RARA Antibody

CATALOG NUMBER: 46-267

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SPSLSPSSHRSSPATQSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 50kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RARA
Additional Names:
RARA, RAR, NR1B1, retinoic acid receptor, alpha, Retinoic acid receptor, alpha polypeptide, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form
Protein Accession Number:
NP_000955.1,NP_001138773.1,NP_001019980.1,NP_001138774.1
Protein Gi Number:
4506419
Ncbi Gene Id Number:
5914
Background Reference 1:
Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R. Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity. J Natl Cancer Inst. 2005 Jan 5;97(1):21-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-267

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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