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Western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of RARA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
RARA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).

RARA Antibody

CATALOG NUMBER: 57-306

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RARA
Additional Names:
Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1
Protein Accession Number:
P10276
Protein Gi Number:
133483
Ncbi Gene Id Number:
5914
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene represents a nuclear retinoic acid receptor. Theencoded protein, retinoic acid receptor alpha, regulatestranscription in a ligand-dependent manner. This gene has beenimplicated in regulation of development, differentiation,apoptosis, granulopoeisis, and transcription of clock genes.Translocations between this locus and several other loci have beenassociated with acute promyelocytic leukemia. Alternatively splicedtranscript variants have been found for this locus.
Background Reference 1:
Park, U.H., et al. J. Biol. Chem. 285(44):34269-34278(2010)
Background Reference 2:
Asleson, A.D., et al. Cancer Genet. Cytogenet. 202(1):33-37(2010)
Background Reference 3:
Catalano, A., et al. Blood 110(12):4073-4076(2007)
Background Reference 4:
Wells, R.A., et al. Nat. Genet. 17(1):109-113(1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-306

List Size:
400 ul

List Price:

$519.00

Datasheet
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