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Western blot analysis in K562 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with RARB Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.
Western blot analysis in K562 cell line lysates (35ug/lane).

RARB Antibody

CATALOG NUMBER: 59-680

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the Central region of human RARB.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RARB
Additional Names:
Retinoic acid receptor beta, RAR-beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon, RARB, HAP, NR1B2
Protein Accession Number:
P10826
Protein Gi Number:
17380507
Ncbi Gene Id Number:
5915
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes retinoic acid receptor beta, a member ofthe thyroid-steroid hormone receptor superfamily of nucleartranscriptional regulators. This receptor localizes to thecytoplasm and to subnuclear compartments. It binds retinoic acid,the biologically active form of vitamin A which mediates cellularsignalling in embryonic morphogenesis, cell growth anddifferentiation. It is thought that this protein limits growth ofmany cell types by regulating gene expression. The gene was firstidentified in a hepatocellular carcinoma where it flanks ahepatitis B virus integration site. The gene expresses at least twotranscript variants; one additional transcript has been described,but its full length nature has not been determined. [provided byRefSeq].
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010)
Background Reference 3:
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Background Reference 4:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-680

List Size:
400 ul

List Price:

$495.00

Datasheet
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