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Western blot analysis of extracts of various cell lines, using RARRES2 antibody (22-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15min.
Immunohistochemistry of paraffin-embedded rat kidney using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RARRES2 antibody (22-590). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using RARRES2 antibody (22-590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15min.

RARRES2 Antibody

CATALOG NUMBER: 22-590

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 17-163 of human RARRES2 (NP_002880.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
BT-474
Positive Control 3:
HeLa
Positive Control 4:
Mouse liver
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RARRES2
Additional Names:
HP10433, TIG2, retinoic acid receptor responder protein 2, RAR-responsive protein TIG2, chemerin, retinoic acid receptor responder (tazarotene induced) 2, tazarotene-induced gene 2 protein
Ncbi Gene Id Number:
5919
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine and as an antimicrobial protein with activity against bacteria and fungi.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-590

List Size:
100 uL

List Price:

$436.00

Datasheet
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