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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
RARS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of RARS Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

RARS Antibody

CATALOG NUMBER: 55-620

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-634 amino acids from the C-terminal region of human RARS.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RARS
Additional Names:
Arginine--tRNA ligase, cytoplasmic, Arginyl-tRNA synthetase, ArgRS, RARS
Protein Accession Number:
P54136
Protein Gi Number:
20178331
Ncbi Gene Id Number:
5917
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation oftRNA by their cognate amino acid. Because of their central role inlinking amino acids with nucleotide triplets contained in tRNAs,aminoacyl-tRNA synthetases are thought to be among the firstproteins that appeared in evolution. Arginyl-tRNA synthetasebelongs to the class-I aminoacyl-tRNA synthetase family. [providedby RefSeq].
Background Reference 1:
Bottoni, A., et al. J. Cell. Physiol. 212(2):293-297(2007)
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 3:
Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)
Background Reference 4:
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-620

List Size:
400 ul

List Price:

$495.00

Datasheet
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