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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of RARS2 Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with RARS2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using RARS2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

RARS2 Antibody

CATALOG NUMBER: 63-535

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This RARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the C-terminal region of human RARS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RARS2
Additional Names:
Probable arginine--tRNA ligase, mitochondrial, Arginyl-tRNA synthetase, ArgRS, RARS2, RARSL
Protein Accession Number:
Q5T160
Protein Gi Number:
74744409
Ncbi Gene Id Number:
57038
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RARS2 is an arginyl-tRNA synthetase that is found in the mitochondrial matrix.
Background Reference 1:
Edvardson,S., et.al., Am. J. Hum. Genet. 81 (4), 857-862 (2007)
Background Reference 2:
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
Background Reference 3:
Bonnefond,L., et.al., Biochemistry 44 (12), 4805-4816 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-535

List Size:
400 ul

List Price:

$495.00

Datasheet
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