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Ras-GRF1 (phospho Ser916) Antibody

CATALOG NUMBER: 79-801

Clonality:
Polyclonal
Tested Applications:
IHC
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Ras-GRF1 (phospho Ser916) antibody was raised against a peptide sequence around phosphorylation site of Serine 916 (N-E-G (p)-T P) derived from Human Ras-GRF1.
Conjugate:
Unconjugated
Tested Applications:
IHC
Specificity:
The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
Predicted Molecular Weight:
145 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
Rasgrf1
Additional Names:
CDC25, GNRP, GRF1, RASGRF1
Protein Accession Number:
NP_002882.3
Protein Gi Number:
6755288
Ncbi Gene Id Number:
19417
Background:
Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
Background Reference 1:
Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
Background Reference 2:
Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
Background Reference 3:
Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
Background Reference 4:
Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-801

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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