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Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)

RASGRF1 Antibody

CATALOG NUMBER: 63-012

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RASGRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 907-936 amino acids from human RASGRF1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
145 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RASGRF1
Additional Names:
Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, Guanine nucleotide-releasing protein, GNRP, Ras-specific nucleotide exchange factor CDC25, RASGRF1, CDC25, GNRP, GRF1
Protein Accession Number:
Q13972
Protein Gi Number:
13124259
Ncbi Gene Id Number:
5923
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
Background Reference 1:
Iguchi,T., Int. J. Oncol. 31 (2), 285-291 (2007)
Background Reference 2:
Holmes,R., Mol. Cell. Biol. 26 (24), 9564-9570 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-012

List Size:
400 ul

List Price:

$495.00

Datasheet
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